The present study aims to limit its scope to explore the three top characters in Vladimir Vladimirovich Nabokov's Lolita through the aforementioned Lacanian concepts. Through the analysis of the main characters in the mentioned novel, this study asserts that these concepts are structured with the effect of the Lacanian symbolic order and the language. In other words, in this study, it is argued that the formation of the human personality takes place in the unconscious, where desire, alienation and sexuality are formed. Moreover, it is laid bare that each character's appreciation of reality hinges heavily on his position in one of Lacanian three Orders.