This book develops a rational design and systematic approach to construct a gene network with desired behaviors. In order to achieve this goal, the registry of standard biological parts and experimental techniques are introduced at first. Then these biological components are characterized by a standard modeling method and collected in the component

General introduction. Survey of synthetic gene networks. Experimental techniques for engineering synthetic gene networks. Mathematical models and design specifications in synthetic gene networks. Robust synthetic biology designs based on system dynamic methods. Construction of promoter library and RBS library for synthesis of gene networks. Robust synthetic gene network designs based on library-search method. Next-generation synthetic gene network.