Molecular Microbiology Laboratory, Second Edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles. - Exposes students to the new molecular-based techniques - Provides faculty with an authoritative, accessible resource for teaching protocols - The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum - Based on a successful course for which the author won a teaching award New to this Edition:- Presents a real-world study of bacterial populations in the environment in the final experiment - Provides an overview of molecular biology in a new review chapter- Demonstrates how to design an experiment and how to interpret the results - Covers grant proposal writing and how panels review proposals - Presents guidance on public speaking and preparing PowerPoint presentations - Includes tutorials on three widely used software packages

Walt Ream, Professor of Microbiology, Oregon State University, received the Richard M. Bressler Senior Faculty Teaching Award for developing the course this book supports. In addition to this work, he has written numerous peer reviewed scholarly papers, review articles, book articles and chapters and has co-authored another book entitled, Molecular Biology Techniques: An Intensive Laboratory Course. His research led to a discovery for which a patent was issued in 2004. He regularly reviews grant proposals for NSF, USDA, DOE, NIH, American Cancer Society, NSERC-Canada, Lindbergh Foundation, Corporation for Plant Biotechnology Research and he reviews papers for Journal of Bacteriology, Plant Molecular Biology, PNAS, Science, Molecular & General Genetics, Microbiological Reviews, Molecular Plant-Microbe Interactions, Biochemistry, Journal of Biological Chemistry, Plasmid, Gene, Plant Physiology, Journal of American Horticulture Society, and Molecular Microbiology. He is a member of Genetics Society of America, American Society for Microbiology, and American Association for the Advancement of Science.

Molecular Microbiology Laboratory, Second edition, is designed to teach essential principles and techniques of molecular biology and microbial ecology to upper-level undergraduates majoring in the life sciences and to develop students' scientific writing skills. A detailed lab preparation manual for instructors and teaching assistants accompanies the lab book and contains a general discussion of scientific writing and critical reading as well as detailed instructions for preparation and peer review of lab reports. Each experimental unit is accompanied by a number of additional writing exercises based upon primary journal articles.

• Exposes students to the new molecular-based techniques
• Provides faculty with an authoritative, accessible resource for teaching protocols
• The only manual to incorporate writing exercises, presentation skills and tools for reading primary literature into the curriculum
• Based on a successful course for which the author won a teaching award

Introduction Experiment 1: Plasmid Purification and Restriction Mapping Experiment 2: Affinity Purification of Histidine-Tagged FnbA Protein Experiment 3: Polymerase Chain Reaction and DNA Sequence Analysis of Bacterial Ribosomal RNA Genes Experiment 4: Terminal Restriction Fragment Length Polymorphism Analysis of 16 S Ribosomal RNA Genes from Uncultured Bacterial Communities Appendix A: Sample Laboratory Reports Appendix B: Sample Proposals Appendix C: Sample Tests Appendix D: Guidance for Presentations Appendix E: Revised Paragraph from J. Biol. Chem. 266: 3811-3814 (1991) Appendix F: Abstract from Appl. Environ. Microbiol. 63: 2647-2653 (1997) Appendix G: Sequence Editing Tutorial Appendix H: Peak Scanner Tutorial Appendix I: Adding Size Standards to Peak Scanner Appendix J: PRIMER Tutorial Appendix K: Checklist for Grading Laboratory Reports Appendix L: Peer Review Checklist Appendix M: Career Advice Appendix N: Molecular Microbiology Laboratory Preparation Manual Bibliography Index