Handbook of Histopathological and Histochemical Techniques (Including Museum Techniques), Third Edition is a reference guide on techniques used in histology and histochemistry, including museum techniques.
This book is comprised of 36 chapters divided into six sections. After giving a brief description of the structure and contents of the cell, this book then turns to the various methods of examination of tissue cells and structures, including the smear method, vital staining, and electron microscopy. The next two sections deal with fixation, decalcification, processing, and section cutting, along with stains, impregnations, and mountants. An exhaustive survey of carbohydrate and protein histochemistry is included. Special procedures such as autoradiography, micro-incineration, and injection techniques are described. The remaining chapters focus on the preparation, mounting, and presentation of museum specimens. The book concludes with an analysis of the microscope.
This monograph will be invaluable to all those with an interest in histology and histochemistry, including undergraduate students in the biological and health sciences, pathologists in both the hospital setting and experimental research, and technologists and workers in various research fields.
Inhalt
Foreword
Preface to Third Edition
Preface to First Edition
Acknowledgments
Part I Introductory
Chapter 1. The Cell
Chapter 2. Methods of Examination of Tissues and Cells
Part II Fixation, Processing and Section Cutting
Chapter 3. Fixation
Chapter 4. Decalcification
Chapter 5. Processing
Chapter 6. Section Cutting
Part III Stains, Impregnations and Mountants
Chapter 7. Theory of Staining
Chapter 8. Preparation of Stains
Chapter 9. Mountants
Chapter 10. Basic Staining and Mounting Procedures
Chapter 11. Routine Stains
Chapter 12. Proteins
Chapter 13. Nucleoproteins-Deoxyribonucleic Acid and Ribonucleic Acid
Chapter 14. Carbohydrates
Chapter 15. Enzymes
Chapter 16. Lipids (Fats, Lipoids, Lipins)
Chapter 17. Pigments
Chapter 18. Micro-Organisms
Chapter 19. Tissues Requiring Special Treatment or Techniques
Chapter 20. Certain Cytoplasmic Constituents and Cell Products
Part IV Special Procedures
Chapter 21. Exfoliative Cytology and Chromosome Techniques
Chapter 22. Autoradiography
Chapter 23. Vital Staining
Chapter 24. Micro-Incineration
Chapter 25. Injection Techniques
Part V Museum Technique
Chapter 26. Preparation, Color Maintenance, Fixation and Storage of Specimens
Chapter 27. Mounting of Museum Specimens
Chapter 28. Special Methods
Chapter 29. Presentation of Museum Specimens
Part VI The Microscope
Chapter 30. The Compound Microscope
Chapter 31. The Dark-Ground Microscope
Chapter 32. Fluorescence Microscopy
Chapter 33. The Polarizing Microscope
Chapter 34. The Phase-Contrast Microscope
Chapter 35. The Interference Microscope
Chapter 36. The Electron Microscope
References
Index