Presents an integrated view of the expression of bacterial genetic information, genome architecture and function, and bacterial physiology and pathogenesis

This book blends information from the very latest research on bacterial chromosome and nucleoid architecture, whole-genome analysis, cell signaling, and gene expression control with well-known gene regulation paradigms from model organisms (including pathogens) to give readers a picture of how information flows from the environment to the gene, modulating its expression and influencing the competitive fitness of the microbe.

Structure and Function of the Bacterial Genome explores the governance of the expression of the genes that make a bacterium what it is, and updates the basics of gene expression control with information about transcription promoter structure and function, the role of DNA as a regulatory factor (in addition to its role as a carrier of genetic information), small RNAs, RNAs that sense chemical signals, ribosomes and translation, posttranslational modification of proteins, and protein secretion. It looks at the forces driving the conservation and the evolution of the dynamic genome and offers chapters that cover DNA replication, DNA repair, plasmid biology, recombination, transposition, the roles of repetitive DNA sequences, horizontal gene transfer, the defense of the genome by CRISPR-Cas, restriction enzymes, Argonaute proteins and BREX systems. The book finishes with a chapter that gives an integrated overview of genome structure and function.

* Blends knowledge of gene regulatory mechanisms with a consideration of nucleoid structure and dynamics

* Offers a 'DNA-centric' approach to considering transcription control

* Views horizontal gene transfer from a gene regulation perspective

* Assesses the opportunities and limitations of designing synthetic microbes or rewiring existing ones

Structure and Function of the Bacterial Genome is an ideal book for graduate and undergraduate students studying microbial cell biology, bacterial pathogenesis, gene regulation, and molecular microbiology. It will also appeal to principal investigators conducting research on these and related topics and researchers in synthetic biology and other arms of biotechnology.

CHARLES J. DORMAN, PhD, ScD, MRIA has held the established chair of Microbiology at Trinity College Dublin, Ireland since 1994. Previously, he held a Royal Society University Research Fellowship at the Biochemistry Department in Dundee University, UK, where he was also a Lecturer in Biochemistry. Professor Dorman is a former Editor-in-Chief of Microbiology, is a Trustee and Council member of the Microbiology Society and has served as the Ambassador to Western Europe of the American Society for Microbiology. He is a founding member of the European Academy of Microbiology, a Fellow of the American Academy of Microbiology, a Fellow of the Royal Society of Biology and a Member of the Royal Irish Academy.

Klappentext

PRESENTS AN INTEGRATED VIEW OF THE EXPRESSION OF BACTERIAL GENETIC INFORMATION, GENOME ARCHITECTURE AND FUNCTION, AND BACTERIAL PHYSIOLOGY AND PATHOGENESIS

This book blends information from the very latest research on bacterial chromosome and nucleoid architecture, whole-genome analysis, cell signaling, and gene expression control with well-known gene regulation paradigms from model organisms (including pathogens) to give readers a picture of how information flows from the environment to the gene, modulating its expression and influencing the competitive fitness of the microbe.

Structure and Function of the Bacterial Genome explores the governance of the expression of the genes that make a bacterium what it is and updates the basics of gene expression control with information about transcription promoter structure and function, the role of DNA as a regulatory factor (in addition to its role as a carrier of genetic information), small RNAs, RNAs that sense chemical signals, ribosomes and translation, posttranslational modification of proteins, and protein secretion. It looks at the forces driving the conservation and the evolution of the dynamic genome and offers chapters that cover DNA replication, DNA repair, plasmid biology, recombination, transposition, the roles of repetitive DNA sequences, horizontal gene transfer, the defense of the genome by CRISPR-Cas, restriction enzymes, Argonaute proteins, and BREX systems. The book finishes with a chapter that gives an integrated overview of genome structure and function.

• Blends knowledge of gene regulatory mechanisms with a consideration of nucleoid structure and dynamics
• Offers a 'DNA-centric' approach to considering transcription control
• Views horizontal gene transfer from a gene regulation perspective
• Assesses the opportunities and limitations of designing synthetic microbes or rewiring existing ones

Structure and Function of the Bacterial Genome is an ideal book for graduate and under- graduate students studying microbial cell biology, bacterial pathogenesis, gene regulation, and molecular microbiology. It will also appeal to principal investigators conducting research on these and related topics and researchers in synthetic biology and other arms of biotechnology.

Inhalt

Preface xiii

1 The Bacterial Genome Where the Genes are 1

1.1 Genome Philosophy 1

1.2 The Bacterial Chromosome 4

1.3 Chromosome Replication: Initiation 6

1.4 Chromosome Replication: Elongation 11

1.5 Chromosome Replication: Termination 12

1.6 Replication Produces Physically Connected Products 13

1.7 Decatenating the Sister Chromosomes 13

1.8 Resolving Chromosome Dimers 14

1.9 Segregating the Products of Chromosome Replication 15

1.10 Polar Tethering of Chromosome Origins 20

1.11 Some Bacterial Chromosomes are Linear 20

1.12 Some Bacteria Have More than One Chromosome 21

1.13 Plasmids 22

1.14 Plasmid Replication 22

1.15 Plasmid Segregation 26

1.16 The Nucleoid 28

1.17 The Chromosome Has Looped Domains 29

1.18 The Macrodomain Structure of the Chromosome 29

1.19 The Chromosome Displays Spatial Arrangement Within the Cell 30

1.20 SeqA and Nucleoid Organisation 31

1.21 MukB, a Condensin-Like Protein 32

1.22 MatP, the matS Site and Ter Organisation 33

1.23 MaoP and the maoS Site 34

1.24 SlmA and Nucleoid Occlusion 34

1.25 The Min System and Z Ring Localisation 34

1.26 DNA in the Bacterial Nucleoid 36

1.27 DNA Topology 36

1.28 DNA Topoisomerases: DNA Gyrase 38

1.29 DNA Topoisomerases: DNA Topoisomerase IV 40

1.30 DNA Topoisomerases: DNA Topoisomerase I 40

1.31 DNA Topoisomerases: DNA Topoisomerase III 41

1.32 DNA Replication and Transcription Alter Local DNA Topology 41

1.33 Transcription and Nucleoid Structure 41

1.34 Nucleoid-associated Proteins (NAPs) and Nucleoid Structure 43

1.35 DNA Bending Protein Integration Host Factor (IHF) 44

1.36 HU, a NAP with General DNA-binding Activity 46

1.37 The Very Versatile FIS Protein 47

1.38 FIS and the Early Exponential Phase of Growth 48

1.39 FIS and the Stringent Response 49

1.40 FIS and DNA Topology 49

1.41 Ferritin-Like Dps and the Curved-DNA-binding Protein CbpA 51

1.42 The H-NS Protein: A Silencer of Transcription 53

1.43 StpA: A Paralogue of H-NS 57

1.44 H-NS Orthologues Encoded by Plasmids and Phage 58

1.45 H-NSB/Hfp and H-NS2: H-NS Homologues of HGT Origin 58

1.46 A Truncated H-NS-Like Protein 59

1.47 Hha-like Proteins 59

1.48 Other H-NS Homologues: The Ler Protein from EPEC …