Extracellular Matrix Influences on Gene Expression emerged from the Second international Santa Catalina Island Colloquium that deals with the discussion on the advances in the molecular biology of extracellular matrices and the epigenetic influences on cell function. Topics discussed include morphological descriptions, genetics, teratology, collagen heterogeneity, and mineralization. The book's 12 chapters discuss the chromosomal and extrachromosomal influences upon transcription, translational and post-translational regulations during development, and regulation of extracellular matrix molecular biosynthesis. The book describes the regulation of tissue-specific collagen biosynthesis and the extracellular matrix macromolecules. It contains papers that explain the outer cell surface specificity and cell interactions. Because there are advances as well in other specific topics, such as the epithelial-masenchymal interactions and extracellular matrix influences on gene expression, this book contains papers that deal with these subject matters. Furthermore, it explains the matrix-cell and cell-matrix interactions and mineralization processes, as well as the aberrations in developmental processes. This book will be useful to those individuals who study the scientific advances in developmental biology.

Photographs of Participants

Participants and Coauthors


Administrative Staff


Preface


Introductory Note


Part I Extracellular Matrix Influences on Gene Expression


1. Craniofacial Anomalies: Past, Present, and Future Priorities for Developmental and Molecular Biology


2. Developmental Role of Intercellular Matrix: Retrospection and Prospection


Part II Chromosomal and Extrachromosomal Influences upon Transcription


3. Studies on Cartilage Differentiation


4. On the Mechanism of Repression and De-repression of Gene Function with 5-Bromo-2'-Deoxyuridine and Dimethyl Sulfoxide


5. Role of Glucocorticoid Receptors in Steroid Induced Protein Synthesis


6. The Control of Chondrogenic and Myogenic Expression in Chick Limb Mesodermal Cells


7. Cell Recognition, Histotypic Adhesion and Enzyme Induction in Embryonic Cells


8. Preparation of Minicells, Microcells and Anucleate Cells and Their Use in Cell Fusion Experiments


9. A New Hypothesis for the Regulation of Gene Expression


Discussion


Part III Translational and Post-Translational Regulation during Development


10. The Structure, Synthesis, and Secretion of Pro-collagen


11. Assembly and Processing of Pro-collagen


12. Possible Control of Collagen Synthesis by Specific Isoaccepting tRNA Species


13. Elaboration of Precursor Collagen by Osteoblasts as Visualized by Radioautography after 3 H-Proline Administration


14. Peptides of Basement Membrane Proteins after Cyanogen Bromide and Potassium Cyanide Cleavage


15. Collagenolytic Activity in Human Platelets


16. The Effects of Homologous and Heterologous tRNA on the Synthesis of Submaxillary Gland Proteins in vitro


Discussion


Part IV Regulation of Extracellular Matrix Macromolecular Biosynthesis


17. Chondrogenic Differentiation in Cultured Somites


18. The Influence of External Potassium on the Accumulation of Matrix Components by Chondrocytes in vitro


19. Control of Corneal Differentiation in vitro by Extracellular Matrix


20. Role of Enzyme-Enzyme Interactions in the Organization of Multi-Enzyme Systems


21. Hyaluronic Acid-Proteoglycan Interaction and the Influence of Hyaluronic Acid on Proteoglycan Synthesis by Chondrocytes from Adult Cartilage


22. The Relation of Phagocytosis to the Secretion of Non-Lysosomal Enzymes by Connective Tissue Cells


23. The Regulation of Collagen Fibril Formation


24. Epithelial-Specific Extracellular Matrix Influences on Mesenchyme Collagen Biosynthesis in vitro


25. Generation of Cell Diversity


Discussion


Part V Regulation of Tissue-Specific Collagen Synthesis


26. Biosynthesis of Type III Collagen: Relative Insolubility of the Molecules Synthesized in the Presence of a Lathyrogen


27. Biosynthesis of Collagen by Chondrocytes in vitro


28. The Biosynthesis of Type II Collagen (Cartilage) During Limb Morphogenesis and Regeneration


29. Collagen of Rat Skin and Bovine Articular Cartilage: Their Hydrodynamic Properties During Interactions with Proteoglycans


30. Intracellular Transport and Secretion of Pro-collagen in Chick Bone as Shown by E.M. Radioautography and Biochemical Analysis


31. Collagen Fibrillogenesis in vitro and in vivo: The Existence of Unique Aggregates and the Special State of the Fibril End


32. Proteoglycan-Collagen Interaction: Possible Developmental Significance


33. The Existence of Chemically Distinct Soluble Collagens in Prenatal and Post-natal Bovine Skin and Tendon


Discussion


Part VI Extracellular Matrix Macromolecules


34. Proteins of the Periodontium: The Chain Structure of the Collagens of Bovine Cementum and Periodontal Ligament


35. Some Electrostatic Properties of Reconstituted Collagen Fibrils


36. Enamel Proteins: Identification of Epithelial-Specific Differentiation Products


37. Glycosaminoglycan Conformations


38. Keratan Sulfate of Cartilage and Nucleus Pulposus


39. Organization of Extracellular Matrix in Bovine Articular Cartilages


40. The Structure of Cartilage Proteoglycans


41. Regulation of Epiphyseal Cartilage Maturation


Discussion


Part VII Outer Cell Surface Specificities and Cell Interactions


42. Tissue-Specific Differences and Membrane Site Mobility in Intercellular Adhesion


43. Influence of Antigenic Matrices on the Antibody Response


44. The Location and Nature of Molecules Potentially Involved in Morphogenesis of the Developing Nervous System


45. Lectins: Probes for Cell Surface Structure and Architecture


46. The Influence of Tissue Interactions and Extracellular Macromolecules on the Control of Phenotypic Expression and Synthetic Capacity of Bone and Cartilage


Discussion


Part VIII Epithelial-Mesenchymal Interactions


47. How Are Embryonic Preameloblasts Instructed by Odontoblasts to Synthesize Enamel?


48. Transmission and Spread of Kidney Tubule Induction


49. Control of DNA Synthesis in the Embryonic Pancreas: Interaction of the Mesenchymal Factor and Cyclic AMP


50. Epithelial-Mesenchymal Interactions Between Developing Chick Epidermis and Rabbit Embryo Mammary Mesenchyme


51. On Odontogenic Tissue Interactions


52. Tissue Environmental Influences on the Development of Melanoblasts in Steel Mice


53. Epithelial Cell Differentiation During Secondary Palate Development


Discussion


Part IX Extracellular Matrix Influences on Gene Expression


54. Extracellular Factors Controlling the Migration and Differentiation of the Ganglioblasts of the Autonomie Nervous System


55. Collagen Synthesis in Cultured Neural Crest Cells, Their Derivatives and Retinal Pigmented Epithelium: Stimulation of (a1)3 Collagen Production


56. A Substratum of Bone Matrix Gelatin for Chondrogenesis in Tissue Culture and in vivo


57. Collagenous Bone Matrix and Gene Expression in Fibroblasts


58. Bone Formation in HeLa Cell-Mouse Thigh Tumors Expla…