Separation Methods in Organic Chemistry and Biochemistry aims to provide perspectives for the commonly used separations methods and to discuss indications for their use. The book discusses the determination of molecular properties useful in separation based on micro test methods, paper chromatography, thin-layer chromatography, and electrophoresis. The text then describes the theoretical principles of group-separation procedures, liquid-liquid partition, ion-exchange selectivity, gel permeation, and adsorption. Methods of influencing the selectivity coefficients, the basic theory of fractionation methods, and the principles of application are also encompassed. Biochemists and chemists will find the book useful.

Preface
Introduction

I. Types of Separation Considered

II. Distribution Coefficient

III. Use of Micro Methods

IV. Evaluation of Separation Processes

I. General Principles

I. Stability

II. Group Separations

III. Fractionation Separations

References

II. Determination of Molecular Properties

I. Introduction

II. Chromatographie Procedures

III. Micro Tests

References

III. Solvent Extraction

I. Partition Ratio

II. Application of Solvent Extraction for Group Separation

III. Application of Solvent Extraction for Fractionation Separation

IV. Partition Chromatography

V. Countercurrent Distribution

References

IV. Gel Filtration and Gel-Permeation Chromatography

I. Mechanism

II. Substances Used for Gel Separations

III. Column Performance

IV. Factors Influencing Kd

V. Applications of Gel Filtration

VI. Applications of Gel-Permeation Chromatography

References

V. Ion Exchange

I. General Discussion

II. Ion Exchange Applications

References

VI. Adsorption Processes

I. General Principles

II. Application of Adsorption Procedures

References

Appendix I. Dielectric Constants of Some Common Solvents (at 25°C)

Appendix II. Ion Exchange Resins

Appendix III. General Thin-Layer and Paper Chromatographie Techniques

Appendix IV. Conversion Table for Rf and Rm

Author Index

Subject Index