In situ hybridization is a technique that allows for the visualization of specific DNA and RNA sequences in individual cells, and is an especially important method for studying nucleic acids in heterogeneous cell populations. in situ Hybridization in Electron Microscopy reviews the three main methods and describes the different stages in detail: the preparation of tissue, pretreatment, hybridization, and visualization of the hybridization products. The book includes principles of methodology and practical protocols, along with specific comments, in a two-column user-friendly format. With nearly 150 figures and tables, this book is an essential companion for any researcher in this field.

Gerard Morel

Annie Cavalier

Lynda Williams

General Introduction. Abbreviations. Probes. Principles of Methodology. Sample Preparation. Post-Embedding Technique. Pre-Embedding Technique. Frozen Tissue Technique. Semithin Sections. Controls and Problems. Examples of Results. Appendices. Glossary. Index.