Although the polymerase chain reaction has revolutionized genetic analysis by amplifying rare nucleic acid sequences, the in situ application is the only method that allows the localization of amplified signal within tissue structure. The applications of in situ polymerase chain reaction have greatly enhanced the field of investigation in many disc

Gérard Morel, Ph.D., D.Sc., is a research director at the National Center of Scientific Research (CNRS), at University Claude Bernard-Lyon 1, Villeurbanne, France. Mireille Raccurt, Ph.D., is an engineer in biology in a laboratory of the CNRS (Molecular Physiology), at Claude Bernard-Lyon 1 University, Villeurbanne, France.

General Introduction Chapter 1 - General Principles Chapter 2 - Preparation of Samples Chapter 3 - Pretreatments Chapter 4 - Reverse Transcription (RT) Chapter 5 - Polymerase Chain Reation (PCR) Chapter 6 - Hybridization Chapter 7 - Revelation Chapter 8 - Electron Microscopy Chapter 9 - Controls and Problems Chapter 10 - Typical Protocols Chapter 11 - Examples of Observations