Ultracentrifugation of Macromolecules: Modern Topics covers the theoretical description of the problem of sedimentation equilibrium of polydisperse nonideal solutions. The book discusses the sedimentation equilibrium in polydisperse nonideal solutions and the size distribution analysis by ultracentrifugal methods. The text also describes the simultaneous sedimentation and equilibrium in beta-lactoglobulin B, chymotrypsinogen A, and lysozyme solutions; as well as the sedimentation analysis of a multiple myeloma G-globulin. People involved in physical biochemistry will find the book invaluable.
Inhalt
Foreword
Preface
Acknowledgements
List of Symbols
Part 1 Polydisperse Solute Systems
Chapter I. Sedimentation Equilibrium in Polydisperse
Nonideal Solutions
History
Theory
Sedimentation Equilibrium Experiments
Summary
References
Chapter II. Size Distribution Analysis by Ultracentrifugal Methods
Theory
Experiment I. Polystyrene-Cyclohexane at 34.2°C
Experiment II. Dextran-Water at 25°C
Summary
References
Part 2. Self-Association Reactions in Protein Systems
Chapter III. Simultaneous Sedimentation and Chemical Equilibrium in ß-Lactoglobulin B, Chymotrypsinogen A, and Lysozyme Solutions
ß-Lactoglobulin B
Lysozyme
Chymotrypsinogen A
Summary
References
Chapter IV. Sedimentation Analysis of a Multiple Myeloma G-Globulin
Theory
Experimental Results
Correlation of the Data
Summary and Concluding Remarks
References
Part 3 Appendixes
Appendix A. A Brief Introduction to the Theory of Ultracentrifugai Analysis
Basic Principles
Sedimentation Equilibrium
Sedimentation Transport
References
Bibliography
Appendix B. Molecular Homogeneity and Its Demonstration
Sedimentation Velocity
Sedimentation Equilibrium
References
Author Index
Subject Index