Building from general principles, the authors clearly explain the fundamental role of epithelia in plasma electrolyte and water balance. Emphasis is placed on experimental approaches and methodology. A comprehensive glossary of terms is included.
Inhalt
Epithelial structure and function. Epithelial transport proteins. Epithelial polarity. Characterization of epithelial ion transport. Epithelial electrophysiology. Impedance analysis of epithelia. Measurements and interpretation of cytoplasmic ion activities. Measurements of cell volume. Methods and experimental analysis of isolated epithelial membranes. Methods and experimental analysis of single ion channels. Epithelial cell culture. Measurements and interpretation of cytoplasmic ion activities. Signalling pathways regulating ion transport in polarized cells. The cytoskeleton and epithelial function. Future perspectives: molecular biology and pathophysiology.