Membrane Glycoproteins: A Review of Structure and Function deals with membrane glycoproteins found in biological systems. The book describes the structure and biosynthesis of the glycoproteins in relation to known or postulated functions in membranes.
The text opens with an introduction and a topic on detection and distribution of membrane glycoproteins. The book then notes that the isolation of membrane glycoproteins brought by the progress in research and technology of membrane solubilization and purification of the soluble components is now possible. Discussion is also directed to glycoproteins as being integral components of intracellular membranes, and not just located on cell surfaces. Through the structural analysis of glycoproteins produced by the secretory glands, analysis of human blood group antigens is available. Likewise, discoveries are made, explaining that lectins are useful reagents in detecting the type and numbers of glycoproteins found on cellular members. Lectins are likewise being widely used in tests for carbohydrate-containing substances in membrane-mediated processes. The metabolism, growth control, and cell surface reactions of membrane glycoproteins are also explained.
The book can serve as a guide for biologists, chemists, biochemists, and academicians interested in the study of membranes or glycoproteins.
Inhalt
1. Introduction: Membranes and Membrane Glycoproteins
2. Detection and Distribution of Membrane Glycoproteins
Histochemical Evidence
Histochemistry of Isolated Membranes
Chemical Evidence
Cell Coats, Glycocalyx, 'Fuzz' and Membrane Glycoproteins
Molecular Dimensions of Membrane Glycoproteins
3. Isolation of Membrane Glycoproteins
Introduction
Solubilisation Methods
Separation and Purification Methods
Polyacrylamide Gel Electrophoresis of Membrane Glycoproteins
Erythrocyte Membranes
KB Cell Membranes
4. Structure of Membrane Glycoproteins
A Model
Erythrocyte Membrane Glycoproteins
Cytochrome B5 and Cytochrome B5 Reductase
Summary and Additional Evidence
Surface Labelling of Membrane Glycoproteins
5. Intracellular Membrane Glycoproteins
Introduction
Sarcoplasmic Reticulum
Lysosomes
Mitochondria
Nuclei
Zymogen Granules
Milk Fat Globule Membrane
6. Membrane Glycoproteins as Antigens
Antigens of The ABO System
Antigens of The MN System
Strong Histocompatibility Antigens
7. Lectins
Properties of Lectins
Glycoprotein Receptors For Lectins
Lectin Cytotoxicity and Selection of Resistant Cells
Reaction of Lectins With Cells
Lectins as Histochemical Reagents
8. Lymphocyte Membrane Glycoproteins
Lymphoid Cells
Lymphocyte Membranes
Clusters and 'Cap' Formation
Biological Implications In Lymphocyte Transformation
Transformation of Lymphocytes Induced By Oxidation of Membrane Glycoproteins
9. The Mobility of Membrane Glycoproteins
Membrane Fluidity-A General Property of Cells
Membrane-Intercalated Particles
Cell Agglutination
10. Biosynthesis of Glycoproteins
Intermediary Metabolism and Sugar Labelling In Whole Cells
Glycosyl Transferases
Assembly of Carbohydrate Chains
Membrane Flow In Glycoprotein Biosynthesis
Fidelity of Glycoprotein Biosynthesis
Lipid Intermediates In Glycoprotein Biosynthesis
Control of Glycosyl Transferases
11. Metabolism of Membrane Glycoproteins
Biosynthesis of Membrane Glycoproteins and Membrane Biogenesis
Surface Membrane Turnover
12. Membrane Glycoproteins and Growth Control
Cellular Regulation of Membrane Glycoprotein Biosynthesis
Regulation of Membrane Glycoprotein Biosynthesis In Neoplastic Cells
Membrane Glycoproteins In Cells With Finite Life Span In Culture
13. Membrane Glycoproteins and Cell Surface Reactions
Surface Located Enzymes of Glycoprotein Metabolism
Roles In Adhesion
Roles In Glycoprotein Catabolism
Glycoprotein Structure and The Specificity of Synaptic Connections
Appendix A: Terminal Sugar Sequences of Membrane Glycoproteins In Nervous Tissue
Appendix B: Glycoprotein Components of Myelin
References
Index