A Manual of Paper Chromatography and Paper Electrophoresis provides a comprehensive discussion of the techniques of paper chromatography and paper electrophoresis. The book is organized into two parts. Part I on paper chromatography provides a readily accessible source for some of the many uses and adaptations of paper chromatography. An effort has been made to write a practical manual in which tried and proved procedures, employing relatively simple equipment and available reagents, are summarized. Part II on paper electrophoresis discusses basic principles and methodology. The emphasis throughout has been on the separation of protein mixtures, particularly blood serum. This reflects the fact that it is in this particular application that paper electrophoresis has thus far not been challenged by paper chromatography, whereas many of the smaller molecules can be resolved equally well or better by the thus far more widely employed chromatographic procedures.

Preface

Part I. Paper Chromatography


I. Introduction


II. Theory of Paper Chromatography


III. General Methods


IV. Quantitative Methods


V. Amino Acids, Amines, and Proteins


Section I: Amino Acids and Peptides


Section II: Amino Acid Derivatives


Section III: Amines


Section IV: Separation of Proteins


VI. Carbohydrates


Section I: General Procedure


Section II: Simple Sugars and Disaccharides


Section III : Oligo- and Polysaccharides


Section IV: Sugar Phosphates and Other Substituted Sugars


Section V: Miscellaneous Derived Sugars


VII. Aliphatic Acids


Section I: Volatile Aliphatic Acids (C1 - C9)


Section II: Non-Volatile Water-Soluble Aliphatic Acids


Section III: a-Keto Acids


Section IV: Higher Fatty Acids


Section V: Miscellaneous Lipids


VIII. Steroids and Bile Acids


Section I: Steroids


Section II: Bile Acids


IX. Purines, Pyrimidines and Related Substances


Section I: General Directions


Section II: Detailed Experimental Procedures


X. Phenols, Aromatic Acids, andPorphyrins


Section I: Phenols and Aromatic Acids


Section II: Porphyrins


XI. Miscellaneous Organic Substances


Alcohols


Ketones and Aldehydes


Sulfa Drugs


Barbiturates


Adrenaline and Related Compounds


Alkaloids


Chlorogenic and Caffeic Acids


Nicotinic Acid and Related Compounds


Color Reagent for Isonicotinoyl Hydrazide


Tryptophan and Indoleacetic Acid Derivatives


Imidazoles


Urea, Creatine, Creatinine, etc.


Methylol Compounds


Thioureas


Phospholipids and Their Nitrogenous Constituents


Miscellaneous Organic Bases


Chloroplast, Anthraquinone, and Other Pigments


Coumarins and Related Compounds


Flavonoid Pigments


Tannins


Acridines


Flavin Derivatives


Natural Pterins and Synthetic Derivatives


pH Indicators and Other Dyes


Coal Tar Colors


Photographic Developers


Organic Insecticides


Polyamides (Nylons)


Natural Rubber and Resins


Naphthoquinones


Guanidine Derivatives


XII. Antibiotics and Vitamins


Section I: Antibiotics


Section II: Vitamins


Section III: Plant Growth Substances


XIII. Inorganic Separations


Part II. Paper Electrophoresis


Introduction


XIV. General Theory


Adsorption


Partition Chromatography


Membrane


Polarization


XV. Methods


General Considerations


Closed Strip Method


Open Strip Techniques


Hanging Strip Method


Summary


XVI. Two Dimensional Technique


Closed Sheet Method


Hanging Sheet Method


Combination of Electrophoresis and Chromatography


XVII. Continuous Electrophoresis


XVIII. Some Quantitative Considerations


Appendix


Electrolytes Commonly Employed in Paper Electrophoresis


Dyeing


Procedures


Bibliography


Part I


Part II


Author Index


Subject Index