Paper Chromatography: A Laboratory Manual focuses on methods, technologies, and processes, and aims to provide readers with a readily accessible source for the uses and adaptations of paper chromatography.
The book first offers information on general methods, including descending, ascending, and ascending-descending chromatography, filter paper ""chromatopile"", ""reversed phase"" paper chromatography, and paper electrophoresis. The text then elaborates on quantitative methods and amino acids, amines, and proteins. Discussions focus on visual comparison, elution, area of spot, total color of spot, maximum color density, identification of amines, separation of proteins, and general directions.
The publication examines carbohydrates and aliphatic acids and steroids. Topics include simple sugars, miscellaneous derived sugars, and aliphatic acids. The text also ponders on purines, pyrimidines, and related substances and phenols, aromatic acids, and porphyrins.
The text is a valuable reference for readers interested in paper chromatography.
Inhalt
Foreword
I. Introduction
II. Theory
III. General Methods
Method I: Kapillaranalyse
Method II: Descending Chromatography
Trough
Chromatograph Chamber
Experimental Procedure
Method III: Ascending Chromatography
Chromatograph Chamber
Filter Paper Rack
Experimental Procedure
Comments
Method IV: Ascending Descending Chromatography
Chromatograph Chamber
Comments
Method V: Filter Paper "Chromatophile"
Method VI: "Reversed Phase" Paper Chromatography
Method VII: Circular Filter Paper Chromatography
Apparatus
Experimental Procedure
Identification of Zones
Method VIII: Microbiological and Enzymatic Methods
Method IX: Paper Electrophoresis
Paper Chromatography in an Electrical Field
Paper Electrophoresis
Experimental Technique
Effects of Temperature and Saturation on Paper Chromatograms
Filter Paper
Application of Sample to Paper
Micropipettes
Application of Sample
Elution of Spots
Photoelectric Apparatus
Densitometers
Ultraviolet Spectrophotometer
IV. Quantitative Methods
Method I: Visual Comparison
Visual Comparison Method after Berry and Cain
Method II: Elution
Elution Technique after Fowden, Boissonas, and Block
Method III: Area of Spot
Comment
Method IV: Total Color of Spot
Total Color Density Modified from Block and Bull
Total Color Density According to Rockland
Comment
Method V: Maximum Color Density
Maximum Color Density on Two-Dimensional Paper Chromatograms
Maximum Color Density on One-Dimensional Paper Chromatograms
Method VI: Area Density Method
Method VII: Retention Analysis
V. Amino Acids, Amines and Proteins
Section I: Amino Acids and Peptides
General Directions
Section II: Identification of Dinitrophenyl Amino Acids and Amino Alcohols
Dinitrophenyl Derivatives
Amino Alcohols
Section III: Identification of Amines
?? LEVELLING
Solvents
Paper
Quantities Used
Color Reagents
Preparation of Sample
Comments
Section IV: Separation of Proteins
Conventional Chromatography
Paper Electrophoresis
VI. Carbohydrates
Section I: Simple Sugars
General Directions
Experimental Procedure
Section II: Di- and Polysaccharides, Starch and Cellulose
Preparation of Samples
Detection of Spots
Section III: Substituted Sugars
General Directions
Section IV: Miscellaneous Derived Sugars
Sugar Acids
Polyhydric Alcohols
VII. Aliphatic Acids and Steroids
Section I: Aliphatic Acids
General Directions
Detailed Experiment
Section II: a-Keto Acids
General Directions for a-Keto Acids
Section III: Steroids
General Directions
Detailed Experiment
VIII. Purines, Pyrimidines, and Related Substances
General Directions
Quantities Used
Paper
Solvents
Techniques Used
Preparation of Samples
Detection and Quantitative Evaluation of Spots
IX. Phenols, Aromatic Acids, and Porphyrins
Section I: Phenols and Aromatic Acids
General Directions
Detailed Experiments
Section II: Porphyrins
General Directions
Detailed Experiment
X. Miscellaneous Organic Substances
Experimental Details
Adrenaline and Related Compounds
Lignin Degradation Products
Choline, Ethanolamine and Serine
Alkaloids
Nicotinic Acid and Related Compounds
ß-Indole Acetic Acid and Tryptophan
Anthraquinone Pigments
pH Indicators
Flavonoid Pigments
Chlorinated Organic Insecticides
Pterins
Urea, Creatine, Creatinine, and Miscellaneous Compounds
XI. Antibiotics and Vitamins
Section I: Antibiotics
General Directions
Detailed Experiments
Section II: Vitamins
General Directions
Experimental Details
XII. Inorganic Separations
General Directions
Quantities Used
Paper
Solvents
Special Techniques Used
Preparation of Samples
Detection and Determination
Experimental Details
Comments
Bibliography
Author Index
Subject Index