Skeletal muscle cell differentiation is exogenously regulated by cytokines and growth factors and it is post-transcriptionally regulated by microRNAs (miRNAs). This study presents miRNA and gene expression data of differentiating skeletal muscles with or without concomitant TNF-alpha or IGF1 exposure to elucidate the function of miRNAs. Results indicate specific miRNA-target relations during skeletal muscle differentiation and show that miRNAs mediate the effect of TNF-alpha.