Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enablesthe reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.
Coming Soon in the Fungal Biology series:
Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens
Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites
Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2
van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi
Schmoll, Dattenbock / Gene Expression Systems in Fungi
Dahms / Advanced Microscopy in Mycology
Autorentext
Editors
Vijai Kumar Gupta is Assistant Professor of Biotechnology at MITS University, India. Presently, he is working as a Post Doctoral Research Scientist, at NUIG, Ireland. He obtained his Ph.D. in Microbiology from Dr. R.M.L. Avadh University, India. He has been honoured with several awards, including the prestigious Indian ICAR Senior Research Fellowship and Young Scientist Award-2009, 2011. He has over seven years of research experience in Applied Microbiology and Biotechnology with a special interest in fungal biology. He has edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA.
Dr. Maria G. Tuohy is the Head of the Molecular Glycobiotechnology Research Group, NUI Galway. She has more than 20 years experience in the molecular biochemistry, genetics and biotechnology of fungi, with a special interest in thermophilic ascomycetes and the characterization of these fungi as cell factories for protein production, including novel thermostable enzymes/enzyme systems. Dr. Tuohy has authored 132 research publications. She is also a reviewer for international journals and funding agencies, an associate editor for Microbiology and co-editor of four books with Dr. V. K. Gupta.
Associate Editors
Dr. Manimaran Ayyachamy is a Research Scientist with NUIG, Ireland. He obtained his PhD in Microbiology from the University of Madras, India. He has twelve years of research experience in microbial biotechnology with a special interest in microbial enzymes and biofuels technology. He has co-edited books published from Lambert Academic Publishing, Germany; Nova Science Publishers, USA; Science Publisher, CRC Press, Taylor & Francis, USA; and Springer, USA with Dr. V.K. Gupta
Dr. Kevin M. Turner is a Research Scientist in the division of Manufacturing Sciences and Technology in Pfizer Ireland Pharmaceuticals, Dublin. He obtained his PhD from the University of Limerick, Ireland. He has three years of research experience in microbial biotechnology.
Dr. Anthonia O'Donovan is a Postdoctoral Research Scientist with NUIG, Ireland. She obtained her PhD in Biochemistry from the National University of Ireland Galway, Ireland. She has seven years of research experience in microbial enzymes and biofuels technology.
Klappentext
Laboratory Protocols in Fungal Biology presents the latest techniques in fungal biology. This book analyzes information derived through real experiments, and focuses on cutting edge techniques in the field. The book comprises 57 chapters contributed from internationally recognised scientists and researchers. Experts in the field have provided up-to-date protocols covering a range of frequently used methods in fungal biology. Almost all important methods available in the area of fungal biology viz. taxonomic keys in fungi; histopathological and microscopy techniques; proteomics methods; genomics methods; industrial applications and related techniques; and bioinformatics tools in fungi are covered and complied in one book. Chapters include introductions to their respective topics, list of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting. Each chapter is self-contained and written in a style that enablesthe reader to progress from elementary concepts to advanced research techniques. Laboratory Protocols in Fungal Biology is a valuable tool for both beginner research workers and experienced professionals.
Coming Soon in the Fungal Biology series:
Goyal, Manoharachary / Future Challenges in Crop Protection Against Fungal Pathogens
Martín, García-Estrada, Zeilinger / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites
Zeilinger, Martín, García-Estrada / Biosynthesis and Molecular Genetics of Fungal Secondary Metabolites, Volume 2
van den Berg, Maruthachalam / Genetic Transformation Systems in Fungi
Schmoll, Dattenbock / Gene Expression Systems in Fungi
Dahms / Advanced Microscopy in Mycology
Inhalt
Safety Norms and Regulations in Handling Fungal Specimens.- Methods of Cryopreservation in Fungi.- Long Term Preservation of Fungal Cultures in All-Russian Collection of Microorganisms (VKM): Protocols and Results.- Fungal Specimen Collection and Processing.- Chemical and Molecular Methods for Detection of Toxigenic Fungi and Their Mycotoxins from Major Food Crops.- Identification Key for the Major Growth Forms of Lichenized Fungi.- Microscopic Methods for Analytical Studies of Fungi.- Scanning Electron Microscopy for Fungal Sample Examination.- High Resolution Imaging and Force Spectroscopy of Fungal Hyphal Cells by Atomic Force Microscopy.- Use of Fourier-Transform Infrared (FTIR) Microscopy Method For Detection of Phyto-Fungal Pathogens.- Diagnose of Parasitic Fungi in The Plankton: Technique for Identifying And Counting Infective Chytrids Using Epifluorescence Microscopy.- Fungal Cell Wall Analysis.- Histopathological Technique for Detection of Fungal Infections in Plants.- Development of Media for Growth and Enumeration of Fungi from Water.- Sabouraud Agar for Fungal Growth.- A Method for the Formation of Candida Biofilms in 96 Well Microtiter Plates and its Application to Antifungal Susceptibility Testing.- Screening for Compounds Exerting Antifungal Activities.- Fluorescence In situ Hybridization of Uncultured Zoosporic Fungi.- Staining Techniques and Biochemical Methods for the Identification of Fungi.- Protocol for the In vivo Quantification of Superoxide Radical in Fungi.- Isolation of Intact RNA from Sorted S. cerevisiae Cells for Differential Gene Expression Analysis.- Quantitative PCR Analysis of Double-Stranded RNA-Mediated Gene Silencing in Fungi.- Semi-nested PCR Approach to Amplify Large 8S rRNA Gene Fragments for PCR-DGGE Analysis of Soil Fungal Communities.- Proteomic Protocols for the Study of Filamentous Fungi.- Detection and Quantification of Endo…