Supramolecular Enzyme Organization: Quaternary Structure and Beyond provides a comprehensive discussion of enzyme interactions. It attempts to place conceptually related phenomena in a common perspective by first scrutinizing enzyme interactions at the quaternary level and then moving on towards higher orders of organization. The book begins with a discussion of the hierarchy of enzymes, covering the structure of monomeric enzymes, enzyme structures beyond the monomeric stage, and protein-protein interactions. Separate chapters cover the chemistry of protein associations; the quaternary structure of enzymes; multienzyme systems; multienzyme complexes and conjugates; the association of enzymes with cellular structures; and metabolite compartmentation. The final chapters discuss trends in the study of enzyme systems and the dialectics of enzymology. This book is intended, not for the specialist, who knows much more about the individual questions than the text offers, but rather for the general biochemical reader interested in enzyme organization. There is practically no mathematics involved. Instead, quite elementary things are described, so that students and biologists with less background knowledge on enzymes will be able to join in.
Inhalt
List of Abbreviations
Chapter 1. The Hierarchy of Enzyme Structures
Structure of Monomeric Enzymes
Enzyme Structures Beyond the Monomeric Stage
The Conceptual Framework of Protein-Protein Interactions
Chapter 2. Chemistry of Protein Associations
The Concept of Complementary Surfaces
Complementary Protein Surfaces are Tightly Packed
About the Predominant Role of Hydrophobic Interactions
Electrostatic Effects Mainly Contribute to the Specificity of Protein Associations
Chapter 3. Intramolecular Organization: The Quaternary Structure of Enzymes
Oligomeric Enzymes
Number of Subunits in Oligomeric Enzymes
Subunit Arrangements in Oligomeric Enzymes
Biological Roles of Oligomeric Structure
Quaternary Structure and Enzyme Activity
Quaternary Structure and Protein Stability
Quaternary Structure and Enzyme Regulation
Complex Enzymes
Regulation Through non-Catalytic Subunits
Chapter 4. Intermolecular Organization: Multienzyme Systems
Functional vs. Structural Organization
Functional Consequences of Enzyme Juxtaposition
Proximity Effect
Activation of Physiological and Suppression of non-Physiological Reactions
Coordinate Regulatory Effects
Chapter 5. Multienzyme Complexes and Conjugates
Stable Multienzyme Complexes
Tryptophan Synthase
The a-Ketoacid Dehydrogenase Multienzyme Complexes
Interconvertible Enzymes and Enzyme Cascades
Multienzyme Conjugates
The Arom Multienzyme Conjugate
Fatty Acid Syntheses from Animal Tissues
The Initiation of Pyrimidine Biosynthesis: The Cluster of Carbamoyl-P Synthase-aspartate Carbamoyl Transferase-Dihydroorotase
"Complex U": The Terminator of Pyrimidine Biosynthesis
Aspartokinase I: Homoserine Dehydrogenase I
Other Multienzyme Conjugates
Multienzyme Complex-Conjugates
Fatty Acid Synthase from Yeast
Interactions between "Soluble" Enzymes
Interactions of Glycolytic Enzymes
Interactions between Enzymes of the Mitochondrial Matrix
Chapter 6. Association of Enzymes with Cellular Structures
Enzymes Incorporated into Structures
Integral Membrane Enzymes
Enzymes Loosely Bound to Structures
Glycolytic Enzymes and the Contractile Apparatus
Glycolysis and the Erythrocyte Membrane
The "Glycosome"
Hexokinase and the Mitochondrial Membrane
Theoretical Models of Enzyme Regulation by Reversible Adsorption
Chapter 7. Metabolite Compartmentation
The Cell as Basic Compartment
Intracellular Compartments and Metabolite Pools
Macro-compartments: Plasmatic and non-Plasmatic
Arginine Metabolism in Neurospora: A Case of Regulation via Macro-compartments
Micro-compartments
Metabolite Channeling in Multienzyme Clusters
Dynamic Compartmentation
Heterogeneity of Metabolite Pools Due to Biding Sites
Survey of Methods for the Detection of Metabolite Compartmentation
Chapter 8. Current Trends in the Study of Enzyme Systems
Mathematical Modeling of Enzyme Systems
Study of Enzymes In Vivo
Invasive Methods
Non-Invasive Methods
Systemic Approach to Metabolic Control
Functional Complementarity between Enzymes
Chapter 9. Enzyme Organization: Summary and Perspectives
The Dialectics of Enzymology: Away from and Back to the Living Cell
The Third Dimension of Metabolic Maps
The Dividends of Complexity
Enzyme Organization in Disease and Aging
Enzyme Therapy
Enzyme Technology
Enzymology of the 21st Century
References
Subject Index