Largely driven by major improvements in the analytical capability of mass spectrometry, proteomics is being applied to broader areas of experimental biology, ranging from oncology research to plant biology to environmental health. However, while it has already eclipsed solution protein chemistry as a discipline, it is still essentially an extension

Roger L. Lundblad

Written by a recognized expert, this brilliant work describes the application of chemical modification of proteins to proteomic research, as well as sample preparation, sample prefractionation, analytical technologies, and validation issues in proteomic research. It merges current proteomics concepts into established solution protein chemistry concepts. The author gives particular emphasis to the process by which a biomarker can be identified, validated, and developed into a diagnostic analyte that is useful in clinical applications. According to Proteomics, Lundblad resurrects "the rigorous chemistry literature of the 1950's and 60's, on which most of our present-day knowledge is based."

Preface. A Brief Discussion of Proteomics: Definitions, Concepts, Illusions. An Overview of the Chemical Modification of Proteins. The Application of Chemical Modification to Proteomics: Chemical Proteomics. Sample Preparation for Proteomic Studies. Sample Prefractionation. An Overview of Analytical Technologies Used in Proteomics Research. Clinical Proteomics. Validation Issues in Proteomics and the Transition from Research to Diagnostic Development. References.